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diaminobenzidine dab chromogen reaction  (Vector Laboratories)


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    Vector Laboratories diaminobenzidine dab chromogen reaction
    Diaminobenzidine Dab Chromogen Reaction, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 12458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peroxidase+chromogen/DAB+Peroxidase+(HRP)+Substrate+Kit+(with+Nickel)%2C+3%2C3%E2%80%99-diaminobenzidine/pmc13628094-246-15-24
    Average 96 stars, based on 12458 article reviews
    diaminobenzidine dab chromogen reaction - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Effects of Kisspeptin on rabbit ovulation: a comprehensive study of ovulatory, endocrine and histological response.
    Article Snippet: Mammalian reproductive function is regulated by hypothalamic neurons that secrete Kisspeptin (Kp), a neuropeptide that stimulates gonadotropin-releasing hormone (GnRH) secretion, triggering pituitary gonadotrophins (LH and FSH) and gonadal steroids.. This study evaluated the effect of Kisspeptin-10 (Kp10) on ovulation induction in rabbits, comparing its efficacy with that of the GnRH analogue gonadorelin.. Multiparous New Zealand White × California does were assigned to three groups: control group (0.5 % saline solution, i.v.), GnRH group (20 μg gonadorelin, i.m.), and Kp10 group (250 μg Kp10, i.v.).

    Avidin-Biotin Assay:

    Article Title: Effects of Kisspeptin on rabbit ovulation: a comprehensive study of ovulatory, endocrine and histological response.
    Article Snippet: Mammalian reproductive function is regulated by hypothalamic neurons that secrete Kisspeptin (Kp), a neuropeptide that stimulates gonadotropin-releasing hormone (GnRH) secretion, triggering pituitary gonadotrophins (LH and FSH) and gonadal steroids.. This study evaluated the effect of Kisspeptin-10 (Kp10) on ovulation induction in rabbits, comparing its efficacy with that of the GnRH analogue gonadorelin.. Multiparous New Zealand White × California does were assigned to three groups: control group (0.5 % saline solution, i.v.), GnRH group (20 μg gonadorelin, i.m.), and Kp10 group (250 μg Kp10, i.v.).

    Plasmid Preparation:

    Article Title: Effects of Kisspeptin on rabbit ovulation: a comprehensive study of ovulatory, endocrine and histological response.
    Article Snippet: Mammalian reproductive function is regulated by hypothalamic neurons that secrete Kisspeptin (Kp), a neuropeptide that stimulates gonadotropin-releasing hormone (GnRH) secretion, triggering pituitary gonadotrophins (LH and FSH) and gonadal steroids.. This study evaluated the effect of Kisspeptin-10 (Kp10) on ovulation induction in rabbits, comparing its efficacy with that of the GnRH analogue gonadorelin.. Multiparous New Zealand White × California does were assigned to three groups: control group (0.5 % saline solution, i.v.), GnRH group (20 μg gonadorelin, i.m.), and Kp10 group (250 μg Kp10, i.v.).



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    Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, <t>and</t> <t>Ki-67</t> expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.
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    Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, and Ki-67 expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.

    Journal: bioRxiv

    Article Title: Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs

    doi: 10.64898/2026.04.16.718819

    Figure Lengend Snippet: Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, and Ki-67 expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.

    Article Snippet: Sections were blocked with 3% H2O2, respectively incubated with CK7, TTF-1, Ki-67 and mesothelin primary antibodies at 4°C overnight, followed by secondary antibodies and DAB substate (C09-12, OriGene).

    Techniques: Derivative Assay, Immunohistochemical staining, Staining, Immunohistochemistry, Expressing, Immunofluorescence, Mutagenesis

    Histological and Molecular Concordance Analysis of Tumor Tissue and Organoid. (A) Histological and immunohistochemical concordance of tumor tissue and organoids from Patient 1 and Patient 2. H&E staining shows the overall histological structure, while IHC for CK7, TTF-1, and Ki-67 demonstrates the expression of these markers in both tissue and organoid samples. Scale bars represent 100 μm. (B) Fluorescence microscopy images of Patient 6 organoids. DAPI staining (blue) highlights the nuclei, F-actin staining (red) shows the cytoskeleton, and immunofluorescence for NapsinA, CK7, TTF-1, and Ki-67 (green) indicates the expression and localization of these proteins. Scale bars represent 100 μm. (C) Heatmap showing the correlation between gene expression profiles of tumor (T) and organoid (O) samples from five patients. The color scale ranges from blue (low correlation) to red (high correlation), indicating the similarity in gene expression between corresponding tumor and organoid samples. (D) Mutation profile comparison between tumor (T) and organoid (O) samples. (E) DNA copy number variation (CNV) analysis of Patient 1 and Patient 2 between tumor (T) and organoid (O) samples.

    Journal: bioRxiv

    Article Title: Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs

    doi: 10.64898/2026.04.16.718819

    Figure Lengend Snippet: Histological and Molecular Concordance Analysis of Tumor Tissue and Organoid. (A) Histological and immunohistochemical concordance of tumor tissue and organoids from Patient 1 and Patient 2. H&E staining shows the overall histological structure, while IHC for CK7, TTF-1, and Ki-67 demonstrates the expression of these markers in both tissue and organoid samples. Scale bars represent 100 μm. (B) Fluorescence microscopy images of Patient 6 organoids. DAPI staining (blue) highlights the nuclei, F-actin staining (red) shows the cytoskeleton, and immunofluorescence for NapsinA, CK7, TTF-1, and Ki-67 (green) indicates the expression and localization of these proteins. Scale bars represent 100 μm. (C) Heatmap showing the correlation between gene expression profiles of tumor (T) and organoid (O) samples from five patients. The color scale ranges from blue (low correlation) to red (high correlation), indicating the similarity in gene expression between corresponding tumor and organoid samples. (D) Mutation profile comparison between tumor (T) and organoid (O) samples. (E) DNA copy number variation (CNV) analysis of Patient 1 and Patient 2 between tumor (T) and organoid (O) samples.

    Article Snippet: Sections were blocked with 3% H2O2, respectively incubated with CK7, TTF-1, Ki-67 and mesothelin primary antibodies at 4°C overnight, followed by secondary antibodies and DAB substate (C09-12, OriGene).

    Techniques: Immunohistochemical staining, Staining, Expressing, Fluorescence, Microscopy, Immunofluorescence, Gene Expression, Mutagenesis, Comparison